IADR Abstract Archives
Analyses of Herbal Toothpaste’s Antimicrobial Efficacy: in Vitro Study
Objectives: The aim of this in-vitro study is to evaluate the antibacterial efficacy of different toothpastes on Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Candida albicans, Staphylococcus aureus and Escherichia coli.
Methods: Three conventional toothpastes:
1. Signal Expert Protection (1450 ppm NaF) (Unilever, Bulgaria)
2. Dentasave Klorhex Intensive Care (0,2 % chlorhexidine digluconate) (Drogsan, Turkey)
3. Dentasave Klorhex Daily Care (0,05 % chlorhexidine digluconate) (Drogsan, Turkey)
two herbal toothpastes:
1. Gumgumix (ginger and honey, Beka Ilaç, Turkey)
2. Colgate Hemp Seed Oil (Colgate, Palmolive, USA)
six herbal experimental toothpastes (SPC Kozmetik, Turkey)
1. 5% hemp oil + 5% hemp extract
2. 5% hemp oil + extract+Ag nanoparticle
3. 5% hemp oil + extract+Zn nanoparticle
4. 5% hemp oil + extract+Cu nanoparticle
5. 5% hemp oil + extract+Fe nanoparticle
6. 5% hemp oil + extract+Ag+Zn+Cu+Fe nanoparticle
and one base experimental toothpaste were tested for antimicrobial activity by agar-well diffusion method. Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Staphylococcus aureus, Escherichia coli and Candida albicans strains were prepared at 108 CFU/mL, then spread on agar media. Wells with 6 mm diameter were punched and samples were transferred. Paper discs containing 0.2% chlorhexidine digluconate were used as positive control. After incubation at 37°C for 24-48 hours, the inhibition zones formed around the wells were measured.
Results: It was observed that Klorhex Intensive Care and one experimental toothpaste (containing Ag nanoparticle, hemp oil, hemp extract) are effective against all microorganisms used in the study. The ginger-containing herbal toothpaste didn’t show antimicrobial activity against the microorganisms. Signal Expert Protection, Colgate Hemp Seed Oil and Klorhex Daily Care toothpastes are effective against all microorganisms in the study except E. Coli.
Conclusions: While the addition of hemp oil and extract increased antibacterial activity in experimental toothpastes, Gumgumix toothpaste containing ginger and honey didn’t have an antibacterial effect.
Methods: Three conventional toothpastes:
1. Signal Expert Protection (1450 ppm NaF) (Unilever, Bulgaria)
2. Dentasave Klorhex Intensive Care (0,2 % chlorhexidine digluconate) (Drogsan, Turkey)
3. Dentasave Klorhex Daily Care (0,05 % chlorhexidine digluconate) (Drogsan, Turkey)
two herbal toothpastes:
1. Gumgumix (ginger and honey, Beka Ilaç, Turkey)
2. Colgate Hemp Seed Oil (Colgate, Palmolive, USA)
six herbal experimental toothpastes (SPC Kozmetik, Turkey)
1. 5% hemp oil + 5% hemp extract
2. 5% hemp oil + extract+Ag nanoparticle
3. 5% hemp oil + extract+Zn nanoparticle
4. 5% hemp oil + extract+Cu nanoparticle
5. 5% hemp oil + extract+Fe nanoparticle
6. 5% hemp oil + extract+Ag+Zn+Cu+Fe nanoparticle
and one base experimental toothpaste were tested for antimicrobial activity by agar-well diffusion method. Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Staphylococcus aureus, Escherichia coli and Candida albicans strains were prepared at 108 CFU/mL, then spread on agar media. Wells with 6 mm diameter were punched and samples were transferred. Paper discs containing 0.2% chlorhexidine digluconate were used as positive control. After incubation at 37°C for 24-48 hours, the inhibition zones formed around the wells were measured.
Results: It was observed that Klorhex Intensive Care and one experimental toothpaste (containing Ag nanoparticle, hemp oil, hemp extract) are effective against all microorganisms used in the study. The ginger-containing herbal toothpaste didn’t show antimicrobial activity against the microorganisms. Signal Expert Protection, Colgate Hemp Seed Oil and Klorhex Daily Care toothpastes are effective against all microorganisms in the study except E. Coli.
Conclusions: While the addition of hemp oil and extract increased antibacterial activity in experimental toothpastes, Gumgumix toothpaste containing ginger and honey didn’t have an antibacterial effect.