IADR Abstract Archives
Enamel Demineralization Caused by Various Carbohydrates Challenges -in Vitro Evaluation
Objectives: The main challenge when studying dental caries in-vitro is creating a simple, quick and reproducible model of demineralization. Simplifying in vitro caries models that include multiple factors such as carbohydrate diets, bacteria components and dental products on the formation of caries are needed.
The simplicity and reproducibility of modeling in vitro demineralization of human enamel was evaluated using various liquid carbohydrate diets under the presence of S. mutans UA159 biofilm in-vitro.
Methods: Fifty seven enamel slabs (4x4mm) cut from human extracted teeth were used. A single species S. mutans 72-hour biofilm was created over the enamel surfaces. The specimens were then exposed to two carbohydrate diets (10% sucrose or Keyes #2000), 3 times a day for 15 minutes over the course of four days. pH was measured after each exposure. After four days, viable counts and biofilm biomass were evaluated by CFU/mL and crystal violet staining, respectively. Slabs were photographed using Nikon SMZ25 stereomicroscope before and after exposure to caries promoting conditions. Demineralization was evaluated clinically and by Vickers microhardness test.
Results: Both diets measured equal amounts of enamel demineralization and Vickers microhardness test levels. Viable counts (~109) and biofilm biomass tests presented identical bacterial growth.
Conclusions: The two diets create an equally strong biofilm with similar bacterial growth. Which as presented in the results, lead to the same amount of enamel demineralization. Implying that both diets are good options to choose from while creating in-vitro dental caries using this model. With that said, the enamel demineralization seen was minimal, longer carbohydrate exposures are needed to create enamel caries using this model.
The simplicity and reproducibility of modeling in vitro demineralization of human enamel was evaluated using various liquid carbohydrate diets under the presence of S. mutans UA159 biofilm in-vitro.
Methods: Fifty seven enamel slabs (4x4mm) cut from human extracted teeth were used. A single species S. mutans 72-hour biofilm was created over the enamel surfaces. The specimens were then exposed to two carbohydrate diets (10% sucrose or Keyes #2000), 3 times a day for 15 minutes over the course of four days. pH was measured after each exposure. After four days, viable counts and biofilm biomass were evaluated by CFU/mL and crystal violet staining, respectively. Slabs were photographed using Nikon SMZ25 stereomicroscope before and after exposure to caries promoting conditions. Demineralization was evaluated clinically and by Vickers microhardness test.
Results: Both diets measured equal amounts of enamel demineralization and Vickers microhardness test levels. Viable counts (~109) and biofilm biomass tests presented identical bacterial growth.
Conclusions: The two diets create an equally strong biofilm with similar bacterial growth. Which as presented in the results, lead to the same amount of enamel demineralization. Implying that both diets are good options to choose from while creating in-vitro dental caries using this model. With that said, the enamel demineralization seen was minimal, longer carbohydrate exposures are needed to create enamel caries using this model.