IADR Abstract Archives
Analysing Healthy Versus Decayed Tooth Tissues Using Spectroscopic Methods
Objectives: The aim of this study is to understand the chemical alterations in deep dental and grossly decayed caries in contrast to healthy dentine and enamel.
Methods: Enamel and dentine samples from 20 healthy teeth and 21 carious teeth were collected using a low-speed bur, pooled into vials, and weighed. Samples were ground under liquid nitrogen and divided into sound enamel (ME1), sound dentine (MD1), deep dentine (DDo) and grossly decayed dentine (GDi), respectively. Fourier-transform infrared (FTIR) analysis followed by 13C and 15N nuclear magnetic resonance (NMR) analysis was performed to compare the chemical composition of healthy and decayed tooth material.
Results: FTIR revealed notable differences in the Amide1 (1650 cm-1) and phosphate (1100 cm-1) bands between sound and carious tooth material. Decayed dentine exhibited an increase in the ratio of transmission of amide I / transmission of phosphate in carious as compared to ratio for healthy teeth.
This trend was confirmed by 13C and 15N NMR. Sound enamel has the lowest signal ratio organic carbon/inorganic carbon compared to the corresponding ratio for the dentine tissues (healthy and carious) This signal ratio infers that the protein/mineral content in carious tissues increases with higher degree of decay. The 15N NMR analysis aligned with the 13C NMR and FTIR results.
Conclusions: It is evident from FTIR and 13C,15N NMR analyses that the ratio of the protein and mineral components increases in decayed as compared to healthy teeth.
Methods: Enamel and dentine samples from 20 healthy teeth and 21 carious teeth were collected using a low-speed bur, pooled into vials, and weighed. Samples were ground under liquid nitrogen and divided into sound enamel (ME1), sound dentine (MD1), deep dentine (DDo) and grossly decayed dentine (GDi), respectively. Fourier-transform infrared (FTIR) analysis followed by 13C and 15N nuclear magnetic resonance (NMR) analysis was performed to compare the chemical composition of healthy and decayed tooth material.
Results: FTIR revealed notable differences in the Amide1 (1650 cm-1) and phosphate (1100 cm-1) bands between sound and carious tooth material. Decayed dentine exhibited an increase in the ratio of transmission of amide I / transmission of phosphate in carious as compared to ratio for healthy teeth.
This trend was confirmed by 13C and 15N NMR. Sound enamel has the lowest signal ratio organic carbon/inorganic carbon compared to the corresponding ratio for the dentine tissues (healthy and carious) This signal ratio infers that the protein/mineral content in carious tissues increases with higher degree of decay. The 15N NMR analysis aligned with the 13C NMR and FTIR results.
Conclusions: It is evident from FTIR and 13C,15N NMR analyses that the ratio of the protein and mineral components increases in decayed as compared to healthy teeth.