IADR Abstract Archives
Genetic Polymorphisms of Inflammatory Diseases
Objectives: The amalgamation of new and foundational approaches in human immunogenetics to evaluate mechanisms of disease onset, modes of inheritance, associations, and development, has evolving applications in research. This study aims to evaluate genetic variants for chronic, inflammatory conditions which participate in immune system surveillance evasion to propagate disease progression, by assessing single nucleotide polymorphisms (SNPs) associated with metastatic cancer for oral health variables temporomandibular joint disease (TMD). Discovered genetic associations may serve as biomarkers to aid efforts for preventive measures and interventional support. Comorbid inflammatory conditions categorized as sub-cohorts, will be evaluated to assess confounding impact.
Methods: Approximately 7,000 human profiles from the University of Pittsburgh Dental Registry and DNA Repository be utilized for genomic characterization of 17 SNPs for the following sub-cohorts: periodontitis, obesity, asthma, type II diabetes, periapical lesions, rheumatoid arthritis/autoimmune diseases, and dry socket.
Results: Preliminary data involving catechol-O-methyltransferase (COMT) (rs4818) for asthma, rheumatoid arthritis/autoimmune disease, and periapical lesions have revealed the initial comparison of the frequency of distribution between 3 sub-cohorts (asthma vs. rheumatoid arthritis/autoimmune disease (p=0.91), rheumatoid arthritis/autoimmune disease vs. periapical lesions (p=0.56), asthma vs. periapical lesions (p=0.62)) to reveal there are no statistical differences or effect between disease states. Distribution of the genotypes are in Hardy Weinberg equilibrium (p>0.05) and have a chi-square value with 2 degrees of freedom between 0.01 and 0.9. IL17A (rs3748067) frequency of distribution between sub-cohorts: all combinations have a p-value greater than 0.05, except for type II diabetes vs. dry socket and asthma vs. dry socket. MYO1H, BRINP3, MMP2,9, ADAM10, CA9, IL1-A,B, IL4,10, TGFβ1, AQP5, WNT11, GSK3B, and AXIN2 determinations were also completed.
Conclusions: The study will continue to evaluate the remaining sub-cohorts and SNPs to identify comparative associations between TMD and metastatic cancer gene variants involved in immune system surveillance evasion.
Methods: Approximately 7,000 human profiles from the University of Pittsburgh Dental Registry and DNA Repository be utilized for genomic characterization of 17 SNPs for the following sub-cohorts: periodontitis, obesity, asthma, type II diabetes, periapical lesions, rheumatoid arthritis/autoimmune diseases, and dry socket.
Results: Preliminary data involving catechol-O-methyltransferase (COMT) (rs4818) for asthma, rheumatoid arthritis/autoimmune disease, and periapical lesions have revealed the initial comparison of the frequency of distribution between 3 sub-cohorts (asthma vs. rheumatoid arthritis/autoimmune disease (p=0.91), rheumatoid arthritis/autoimmune disease vs. periapical lesions (p=0.56), asthma vs. periapical lesions (p=0.62)) to reveal there are no statistical differences or effect between disease states. Distribution of the genotypes are in Hardy Weinberg equilibrium (p>0.05) and have a chi-square value with 2 degrees of freedom between 0.01 and 0.9. IL17A (rs3748067) frequency of distribution between sub-cohorts: all combinations have a p-value greater than 0.05, except for type II diabetes vs. dry socket and asthma vs. dry socket. MYO1H, BRINP3, MMP2,9, ADAM10, CA9, IL1-A,B, IL4,10, TGFβ1, AQP5, WNT11, GSK3B, and AXIN2 determinations were also completed.
Conclusions: The study will continue to evaluate the remaining sub-cohorts and SNPs to identify comparative associations between TMD and metastatic cancer gene variants involved in immune system surveillance evasion.