IADR Abstract Archives

Genetic variants associated with neutrophil function in AgP and controls

Objectives: Neutrophil function has been implicated in Aggressive periodontitis (AgP) pathogenesis. Genetic polymorphisms which influence neutrophil function have been proposed as possible risk factors for AgP. The aim of this study was to analyse the association between neutrophil function (phagocytosis and oxidative burst) and genetic variants supposed to affect neutrophil activity in AgP and healthy subjects.

Methods: Twenty patients with a confirmed diagnosis of AgP and twenty healthy controls participated in this study. Neutrophils were extracted from peripheral blood and analyzed in a blind fashion for phagocytosis and oxidative burst by flow cytometry in response to E.coli, two different strains of A.actinomycetemcomitans and P.gingivalis. Blood was also used for DNA extraction and genotyping for the p22phox CYBA 242 C>T and FcãIIa H>R polymorphisms.

Results: The p22phox CYBA 242 T allele was associated with oxidative burst in response to challenge with P. gingivalis (p=0.024). The FcãIIa polymorphism was associated with the phagocytic index (PI) of E. Coli (p= 0.030), but not of periodontopathogenic bacteria. No statistically significant difference was found between AgP patients and controls for PI or oxidative burst values.

Conclusions: This study brings preliminary evidence that genetic factors previously associated with AgP may influence neutrophil function in AgP and healthy individuals.


IADR/AADR/CADR General Session
2009 IADR/AADR/CADR General Session (Miami, Florida)
Miami, Florida
2009
102
Periodontal Research - Pathogenesis
  • Nibali, Luigi  ( UCL Eastman Dental Institute, London, N/A, United Kingdom )
  • O'dea, Mary  ( UCL Eastman Dental Institute, London, N/A, United Kingdom )
  • Bouma, Gerben  ( UCL ICH Molecular Immunology Unit, London, N/A, United Kingdom )
  • Burns, Siobhan  ( UCL ICH Molecular Immunology Unit, London, N/A, United Kingdom )
  • Parkar, Mohamed  ( UCL Eastman Dental Institute, London, N/A, United Kingdom )
  • Donos, Nikos  ( UCL Eastman Dental Institute, London, N/A, United Kingdom )
    • Oral Session
    Periodontal Pathogenesis - Clinical Studies
    04/01/2009