IADR Abstract Archives
Enzymatic Inhibition of Salivary Amylase Using Physalis peruviana Ethanolic Extract
Objectives: Alpha-amylase inhibitors reduce the presence of glucose and it has a particular importance in diabetics patients and possibly in patients with higher risk of dental caries.
The objective of the present study was to determinate the inhibitor effect of Physalis peruviana ethanolic extract rich in galic acid on human salivary alpha-amylase.
Methods: 500 g of healthy fruits were selected, washed with distilled water and then liquefied with 850 ml of ethanol at 96 °for five minutes. They macerated for 48 hours. Subsequently, it was filtered with Whatman 40 paper and dried in an oven at 37 ° C. It was redissolved in 50/50 distilled water / alcohol 96%.
Phenolic compounds were determined using the 1/100 sample and gallic acid such as standard dilutions .To absorbance measuring was used spectrophotometer to 267nm.
Alpha-amylase was contained in human total salivary no stimulated samples those which were obtained from 6 healthy younger patients. To evaluate enzymatic activity each salivary sample was diluted with distilled water (1/50). Samples were divided in 2 groups (control and experimental).
It used a kinetic method at 405nm for the determination using CNP-G3 substrate. The reagent used contained substrate, 5mmol/l calcium chloride, 70mmol/l sodium chloride, 900mmol/l potassium thiocyanate and buffer pH6.
To 1mL of reagent preincubated added 50Ul salivary sample for control group (CG) and 50Ul salivary sample+extract for experimental group (EG). Absorbance was read after 1 and 2 minutes determining the difference between the both and the enzymatic activity.
Results: The concentration of ethanolic extract was 44.28 mg/mL and the gallic acid was 0.0068mg/ml. Bonferroni correction was used for determine between which studied group had a significant difference. The enzymatic activity evaluated was 0.768±2.5U/l for CG and 0.401±3.1U/l for EG which showed significant reduction (P<0.05).
Conclusions: Physalis peruviana ethanolic extract rich in galic acid reduce significantly the enzymatic activity of salivary alpha-amylase samples (P<0.05).
The objective of the present study was to determinate the inhibitor effect of Physalis peruviana ethanolic extract rich in galic acid on human salivary alpha-amylase.
Methods: 500 g of healthy fruits were selected, washed with distilled water and then liquefied with 850 ml of ethanol at 96 °for five minutes. They macerated for 48 hours. Subsequently, it was filtered with Whatman 40 paper and dried in an oven at 37 ° C. It was redissolved in 50/50 distilled water / alcohol 96%.
Phenolic compounds were determined using the 1/100 sample and gallic acid such as standard dilutions .To absorbance measuring was used spectrophotometer to 267nm.
Alpha-amylase was contained in human total salivary no stimulated samples those which were obtained from 6 healthy younger patients. To evaluate enzymatic activity each salivary sample was diluted with distilled water (1/50). Samples were divided in 2 groups (control and experimental).
It used a kinetic method at 405nm for the determination using CNP-G3 substrate. The reagent used contained substrate, 5mmol/l calcium chloride, 70mmol/l sodium chloride, 900mmol/l potassium thiocyanate and buffer pH6.
To 1mL of reagent preincubated added 50Ul salivary sample for control group (CG) and 50Ul salivary sample+extract for experimental group (EG). Absorbance was read after 1 and 2 minutes determining the difference between the both and the enzymatic activity.
Results: The concentration of ethanolic extract was 44.28 mg/mL and the gallic acid was 0.0068mg/ml. Bonferroni correction was used for determine between which studied group had a significant difference. The enzymatic activity evaluated was 0.768±2.5U/l for CG and 0.401±3.1U/l for EG which showed significant reduction (P<0.05).
Conclusions: Physalis peruviana ethanolic extract rich in galic acid reduce significantly the enzymatic activity of salivary alpha-amylase samples (P<0.05).