IADR Abstract Archives
GCF and Salivary HIF-1α, VEGF and TNF-α Levels in Chronic Periodontitis Before and After Periodontal Treatment
Objectives: Hypoxia inducible factor-1α (HIF-1α), an important transcription factor plays active regulatory role in cellular responses, such as angiogenesis, hypoxia and inflammation in several chronic inflammatory diseases. The present study investigated effects of nonsurgical periodontal treatment on the gingival crevicular fluid (GCF) and salivary HIF-1α, vascular endothelial growth factor (VEGF) and tumour necrosis factor (TNF)-α levels as well as on clinical parameters in chronic periodontitis (CP).
Methods: Twenty CP patients and 20 healthy subjects with healthy periodontium were recruited. GCF, saliva samples, and clinical periodontal measurements were recorded at baseline and at 1 and 3 month following therapy. GCF and saliva HIF-1α, VEGF and TNF-α levels were analyzed by using an enzyme-linked immunosorbent assay. Repeated measures of ANOVA was used to test significance of changes within group and for inter-group comparison.
Results: GCF HIF-1α, VEGF and TNF-α levels were significantly higher in CP patients at baseline compared to healthy control (p<0.05). CP patients had higher salivary TNF-α levels compared to healthy (p<0.05), but similar salivary HIF-1α and VEGF levels at baseline (p>0.05). Significant improvements were observed in all clinical parameters after therapy (p<0.05). GCF HIF-1α levels significantly decreased at 1 month, was similar at 3 month compared to baseline, while salivary HIF-1α levels remain unchanged at 1 and 3 months compared to baseline (p>0.05). No significant decrease was observed in GCF and salivary VEGF and TNF -α levels throughout the study period (p>0.05).
Conclusions: The present study evidences for the first time the presence GCF and salivary levels of HIF-1α in chronic periodontitis. Increase in those hypoxia and inflammation markers in CP might indicate the role of hypoxia is often coupled with inflammation in periodontitis pathogenesis. Decreased GCF HIF-1α levels after therapy might suggest that HIF-1 pathway is involved in controlling periodontal inflammatory responses after the resolution of periodontal disease which warrants further investigation.
Methods: Twenty CP patients and 20 healthy subjects with healthy periodontium were recruited. GCF, saliva samples, and clinical periodontal measurements were recorded at baseline and at 1 and 3 month following therapy. GCF and saliva HIF-1α, VEGF and TNF-α levels were analyzed by using an enzyme-linked immunosorbent assay. Repeated measures of ANOVA was used to test significance of changes within group and for inter-group comparison.
Results: GCF HIF-1α, VEGF and TNF-α levels were significantly higher in CP patients at baseline compared to healthy control (p<0.05). CP patients had higher salivary TNF-α levels compared to healthy (p<0.05), but similar salivary HIF-1α and VEGF levels at baseline (p>0.05). Significant improvements were observed in all clinical parameters after therapy (p<0.05). GCF HIF-1α levels significantly decreased at 1 month, was similar at 3 month compared to baseline, while salivary HIF-1α levels remain unchanged at 1 and 3 months compared to baseline (p>0.05). No significant decrease was observed in GCF and salivary VEGF and TNF -α levels throughout the study period (p>0.05).
Conclusions: The present study evidences for the first time the presence GCF and salivary levels of HIF-1α in chronic periodontitis. Increase in those hypoxia and inflammation markers in CP might indicate the role of hypoxia is often coupled with inflammation in periodontitis pathogenesis. Decreased GCF HIF-1α levels after therapy might suggest that HIF-1 pathway is involved in controlling periodontal inflammatory responses after the resolution of periodontal disease which warrants further investigation.