IADR Abstract Archives

Candida species isolated from HIV-infected patients in Ethiopia

Objectives: Infections due to Candida spp. and other fungi have increased dramatically in recent years and are of particular importance because of the rising number of immunocompromised patients. In Ethiopia, there have been no reports about Candida species from oral origin. A rapid screening approach is needed for the diagnosis. The purpose of this study was to determine phenotypic and genotypic identification of Candida species from HIV infected patients in Ethiopia.

Methods: Oral rinses from 13 HIV positive patients were inoculated on CHROMagar. Colonies were then sub cultured on Tobacco agar. DNA was extracted from 71 colonies and amplified by PCR targeting the conserved regions of 18S, 5.8S, and 28S rRNAs. The amplified DNA samples were analyzed their sequence and a part of them were examined by restriction fragment length polymorphism.

Results: Oral Candida colonization was detected in all of patients with or without oral candidiasis. The number of Candida species was more than 1000 CFU/ml in 12 of 13 patients. There were no significant differences of number of candidal colonization between symptomatic and asymptomotic groups. The species isolated most frequently from the patients was C. albicans (12 of 13 patients), followed by C. tropicalis (11/13), C. krusei (9/13), C. parapsilosis (4/13), C. guilliermondi (2/13), C. glabrata (2/13), and C. dubliniensis (2/13). Seven patients had only C. albicans and 1 patient had only C. tropicalis. Two or more Candida species were isolated from the other 5 patients. We showed that phenotypic and genotypic identification process was important to determine the species.

Conclusion: Phenotypic and genotypic identification process was important to determine Candida species. C. albicans, C. tropicalis and C. krusei were major species in Ethiopia. HIV-positive patients have high risk to C. albicans and the other Candida species infection.


World Congress on Preventive Dentistry
2009 World Congress on Preventive Dentistry (Phuket, Thailand)
Phuket, Thailand
2009
152
Posters
  • Isogai, Hiroshi  ( Sapporo Medical University, Hokkaido, N/A, Japan )
  • Isogai, Emiko  ( Health Sciences University of Hokkaido, Hokkaido, N/A, Japan )
  • Mulu, Andargachew  ( Department of Microbiology and Parasitology, College of Medicine and Health Sciences, University of Gondar, Gondar, N/A, Ethiopia )
  • Kassu, Afework  ( Department of Microbiology and Parasitology, College of Medicine and Health Sciences, University of Gondar, Gondar, N/A, Ethiopia )
  • Diro, Ermias  ( Department of Internal Medicine, Faculty of Medicine, Addis Ababa University, Addis Ababa, N/A, Ethiopia )
  • Tekleselassie, Henok  ( Department of Internal Medicine, Faculty of Medicine, Addis Ababa University, Addis Ababa, N/A, Ethiopia )
  • Nishikawa, Takeshi  ( Hokkaido University of Education Sapporo, Sapporo, N/A, Japan )
    • Poster Session
    Other WCPD Topics II
    09/10/2009