IADR Abstract Archives

Inhibitory effect of cationic-steroid-antibiotics on biofilm formation by Streptococcus mutans

Objectives: Cationic-steroid-antibiotics (CSA-13) is a bile-acid-based mimic of endogenous antimicrobial peptides and shares a mechanism of action with many of these antimicrobial agents. Recently, we reported that this compound is bactericidal against cariogenic and periodontopathic bacteria (Oral Microbiol Immunol, 24, 170-172, 2009). In this study, we examined preventive effect of CSA-13 to biofilm production.

Methods: Streptococcus mutans Ingbritt was used for hydroxyapatite (HA) adherence assay. HA squares were coated with or without CSA-13. The bacteria on HA were removed by beads method and cultured on agar plate. Adherent bacteria were confirmed by scanning electron microscopy (SEM).

Results: CSA-13 showed potent adherence inhibitory activity against S. mutans. Number of bacteria on HA with CSA-13 was less than 1.1 X 101. In contrast, number of bacteria on HA without CSA-13 was 2.5 X 105. There are significant differences between groups with and without CSA-13 (P<0.01, paired t test). No bacteria which showed similar size of S. mutans were observed on the surface of non-coated HA, while numerous bacteria were observed on the surface of HA without CSA-13. HA squares promoted growth of S. mutans, whereas HA coated with CSA-13 inhibited the bacterial growth in the medium.

Conclusion: CSA-13 displays adherence inhibition against cariogenic S. mutans. CSA-13 can be useful for modification of dental materials and for prevention of progressive caries. Cationic substances such as CAP18/LL-37 which is a member of cathlelicidin family peptide, could have a function to control biofilm.


World Congress on Preventive Dentistry
2009 World Congress on Preventive Dentistry (Phuket, Thailand)
Phuket, Thailand
2009
86
Posters
  • Isogai, Emiko  ( Health Sciences University of Hokkaido, Hokkaido, N/A, Japan )
  • Isogai, Hiroshi  ( Sapporo Medical University, Hokkaido, N/A, Japan )
  • Okumura, Kazuhiko  ( Health Sciences University of Hokkaido, Hokkaido, N/A, Japan )
  • Savage, Paul B.  ( Brigham Young University, Provo, UT, USA )
    • Poster Session
    Other WCPD Topics I
    09/09/2009