Method: 1. We collected biofilms from tooth surface of three positive subjects of pharyngeal opportunistic bacteria to detect Pseudomonas aeruginosa using fluorescence insitu hybridization (FISH). 2. We analyzed periapical granuloma tissue of 5 subjects to detect Porphyromonas gingivalisby FISH. 3. Peripheral blood samples of 10 subjects at baseline and at the time between 90 s and 5 min. after completion of scaling, 10 min. and 8 hours after completion of scaling were obtained. Blood samples of bacteremia positive subjects treated with bacterial elimination using drug retainer also were collected at the time before and after scaling. Identification of bacteria and measurement of TNF-α in blood at the time before and after scaling were carried out.
Result: 1. FISH analysis showed the positive signal of Pseudomonas aeruginosa in oral biofilms, and suggested that the bacteria were settled in the biofilms. 2. FISH analysis showed the presence of P.gingivalis in periapical granuloma tissue, and colonization of P.gingivalis in the tissue was suggested. 3. α-streptococcus gram-positive cocci and gram-negative bacillus were detected in the peripheral blood of the periodontitis patients collected after scaling. 4. Pathogenic bacteria were not detected in the peripheral blood of the subjects treated with bacterial elimination. The TNF-α values were within the normal reference range.
Conclusion: These findings raise the possibility of controlling odontogenic bacteremia by bacterial elimination using drug retainers. Controlling odontogenic bacteremia will improve systemic vascular condition, and it may be a secondary endpoint of preventive dentistry.