Method: Triclosan methacrylate monomer was synthesized by esterification chemical process. Experimental materials were prepared using (1) TEGDMA - control group, and (2) TEGDMA+30% triclosan methacrylate monomer (TEG-TM). Both groups contained the following photoiniciator system: 2% BAPO and 0.1% BHT . Ten specimens were obtained from each material after photocuring for 40s using the Bluephase curing unit. The antibacterial activity of triclosan methacrylate was evaluated against mature Streptococcus mutans biofilms. Cultures of Streptococcus mutanswere grown for 24 h, adjusted to an optical density (OD550nm) of 1.0, and diluted 20-fold in brain heart infusion broth supplemented with 0.1% sucrose. Biofilms were statically formed during 24 h over the specimen surface. Specimens were washed for 5 min and biofilms disrupted by vortexing. Cell suspensions were serially diluted and plated onto mitis salivarius agar. After incubation for 48 h, cell counting was performed. Four independent experiments were conducted in triplicate. Data were analyzed with ANOVA (p<0.01).
Result: Bacterial counting (log) in the control group (8.7±0.27) was significantly higher than TEG-TM (6.9±0.37). That difference was statistically significant (p<0.01), showing an antibacterial effect against Streptococcus mutansof 1.8 log order reduction.
Conclusion: The triclosan metacrylate monomer demonstrated inhibition effect against Streptococcus mutans when added to TEGDMA and can be added to resinous materials to control the bacterial colonization on material surface.