Methods: Approval from the Medical Ethics Committee of Northwest University for Nationalities was obtained and informed consent was signed before conducting the study. A total of 421 irrelevant individuals in the northwestern part of China, 205 with DMFT≥3 and 216 with DMFT≤2, were recruited into the study. Genomic DNA was extracted from blood by using the reagent kit. Multiplex-PCR and MassARRAY(Sequenom)were used to analyze gene polymorphisms of seven loci on CAVI gene, which include rs227432, rs17032907, rs11576766, rs2274333, rs10864376, rs3765964, rs6680186. Data were analyzed by χ2 test, Haploview 4.2 and SPSS17.0 software package respectively.
Results: Three loci, Rs2274328 (A/C), rs17032907(C/T) and rs11576766 (A/C), located on gene CAVI existed a linkage disequilibrium and their haploid called ACA was related with lower DMFT [P=0.015, OR (95%CI)=0.635(0.440-0.918)]. The frequency of the genotype rs17032907 TT (mutant type) was higher in subjects with high DMFT than that in subjects with low DMFT (p<0.05).
Conclusion: Results of this study showed that genovariation of CAVI was related with the risk of dental caries. Further studies are needed to evaluate the impact of CAVI in caries prevention.
This project was funded by the International Science and Technology Cooperation Projects of Gansu province (2009GS03605).