IADR Abstract Archives

Effect Of Stevia Extract On Cariogenic Traits Of Oral Bacteria

Objective: The natural sweetener stevioside, which is found in the plant Stevia rebaudiana Bertoni, has been used for many years in the treatment of diabetes and obesity. Dental  Caries  is also  associated with frequent ingestion of fermentable sugars. So,  the aim of this work was to investigate the effects of a sweetener containing a stevia extract on pathogenic traits of potential cariogenic bacterial strains.

Method: Experiments were done in vitro on both ATCC and wild strains of Streptococcus mutans and Lactobacillus rhamnosus . The wild strains were isolated from children's saliva.

The antibacterial activity of stevia was examined using a broth dilution method.

The effect on growth was determined by the counting of CFU, after a 24 hours-exposure to the compound.

The inhibition of adherence of growing cells to a glass surface was used to test the anti adhesive activity according to the method described by Hamada and Tori (1978). Positive control was made with sucrose.

The inhibition of acid production was measured by recording the pH variations of the culture medium containing various concentration of stevia extracts. Positive control was made with a glucose containing medium.

Result: Our results show that growth, acid production and adherence capacities of our tested strains were not affected by the stevia extracts.

Conclusion: Sweeteners containing stevia cannot be recommended for the prevention of dental decays.

World Congress on Preventive Dentistry
2013 World Congress on Preventive Dentistry (Budapest, Hungary)
Budapest, Hungary
2013
10
Posters
  • Badet, Marie-cecile  ( U.F.R. of Odontology, Bordeaux Cedex, N/A, France )
  • Mauroux, Marie  ( University Bordeaux Segalen, Bordeaux, N/A, France )
    • Poster Session
    Poster Session I: Effective Oral Disease Prevention and Community Health Promotion - Evidence from Global, Regional and National Programs; Promotion of Oral Health through Common Risk Factor and Population Approaches
    10/10/2013