IADR Abstract Archives
Antibiotic Pastes and Dentin Discoloration: Insights From Microhardness and Spectrophotometric Analysis
Objectives: The aim of this study was to assess the effect of intracanal medications and sealing materials used in regenerative endodontic procedures (REP) on tooth crown’s color and to evaluate their effect on the microhardness of human root dentin at different time intervals.
Methods: Fifty caries-free human premolars were selected for this study. The medicaments tested were: TAP (metronidazole +ciprofloxacin + minocycline), TAP modified (metronidazole+ ciprofloxacin+ amoxicillin), DAP (metronidazole+ ciprofloxacin). A commercial Ca(OH)2 dressing material and non-treated specimens were used for control . Specimens' colors were assessed using a digital spectrophotometer in 1 then 3, 7, 14, 21 and 28 days. Initial and final microhardness measurements were performed using a Shoore microhardness tester. Statistical analysis was performed using SPSS 25.0.
Results: The variation of the initial microhardness was significant between control, TAP modified and DAP. The variation of the final microhardness was very significant between control and all the other treatments. Multiple comparisons showed a significant decrease in lightness for CaOH2 and TAP modified. For the variance of chromaticity from green to red: a *, a significant difference between groups was noted on D1 and D28. As for the chromaticity from blue to yellow: b *,a significant difference between groups was noted in D1, D3 and D28. For the Variance of the color ΔE, The one-way ANOVA showed a non-significant inter- and intra-group difference for the different time intervals.
Conclusions: Our study showed no significant differences in microhardness between dentin treated with Ca(OH)2, TAP, TAPM and DAP. When minocycline was removed (DAP), color change was lower than in TAP and higher than in TAPM groups. Although no statistically significant difference was found between DAP and TAPM, regarding tooth color stability, results suggest that antibiotic replacing is better than its suppression.
Methods: Fifty caries-free human premolars were selected for this study. The medicaments tested were: TAP (metronidazole +ciprofloxacin + minocycline), TAP modified (metronidazole+ ciprofloxacin+ amoxicillin), DAP (metronidazole+ ciprofloxacin). A commercial Ca(OH)2 dressing material and non-treated specimens were used for control . Specimens' colors were assessed using a digital spectrophotometer in 1 then 3, 7, 14, 21 and 28 days. Initial and final microhardness measurements were performed using a Shoore microhardness tester. Statistical analysis was performed using SPSS 25.0.
Results: The variation of the initial microhardness was significant between control, TAP modified and DAP. The variation of the final microhardness was very significant between control and all the other treatments. Multiple comparisons showed a significant decrease in lightness for CaOH2 and TAP modified. For the variance of chromaticity from green to red: a *, a significant difference between groups was noted on D1 and D28. As for the chromaticity from blue to yellow: b *,a significant difference between groups was noted in D1, D3 and D28. For the Variance of the color ΔE, The one-way ANOVA showed a non-significant inter- and intra-group difference for the different time intervals.
Conclusions: Our study showed no significant differences in microhardness between dentin treated with Ca(OH)2, TAP, TAPM and DAP. When minocycline was removed (DAP), color change was lower than in TAP and higher than in TAPM groups. Although no statistically significant difference was found between DAP and TAPM, regarding tooth color stability, results suggest that antibiotic replacing is better than its suppression.