IADR Abstract Archives
Antimicrobial, antifungal and antioxidant activity of a new biologic mouthwash
Objectives: Orthodontic appliances can promote the accumulation of biofilm. Recent research has focused on the use of natural substances from medicinal plants. The aim of this work is to test the antibacterial and antifungal properties, the antioxidant activity as well as the cytotoxicity of a mouthwash formulated with essential oils.
Methods: The antibacterial activity of our mouthwash was tested against strains of Staphylococcus (3 strains: S.aureus ATCC 6538,strain 9Pand 29 P), Streptococci(4strains:oralis,salivarius,anginosus and intermedius), Lactobacilli lactis and Enterococci( 2 strains: E.avium and E.facium) isolated from the oral cavity. to isolate the strains, the samples were cultured on blood agar plates. The antibacterial activity was evaluated by the disk diffusion method, then the method microdilution to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). For the antifungal activity, four strains of Candida were selected: Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida parapsilosis ATCC22019 and Candida krusei ATCC 6258.
For antioxidant activity; we used the DPPH radical reduction test according to Huang method
Results: By testing the antibacterial activity on streptococcal strains, we found inhibition diameters mean=18.5 ±2 mm (SD) using the stock solution. The one in four dilutions also had an anti-streptococcal effect with 14.5±2.3 mm for the mouthwash. This same test was carried out on the strains of S. aureus we found diameters of inhibition between 18 and 20.5 mm testing the mother solution. The C. albicans, C. glabrata, C. krusei and C. parapsilosis strains were sensitive with values between 28.5 ± 0.71mm and 36mm for the stock solution and between 9 and 11mm by testing the dilution to one out of sixteen. the DPPH radical was inhibited by the mouthwash
Conclusions: The tested mouthwash is bactericidal. In addition, we noted a very interesting anti-fungal and antioxidant activity sought from our days, especially for orthodontic patients.
Methods: The antibacterial activity of our mouthwash was tested against strains of Staphylococcus (3 strains: S.aureus ATCC 6538,strain 9Pand 29 P), Streptococci(4strains:oralis,salivarius,anginosus and intermedius), Lactobacilli lactis and Enterococci( 2 strains: E.avium and E.facium) isolated from the oral cavity. to isolate the strains, the samples were cultured on blood agar plates. The antibacterial activity was evaluated by the disk diffusion method, then the method microdilution to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). For the antifungal activity, four strains of Candida were selected: Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida parapsilosis ATCC22019 and Candida krusei ATCC 6258.
For antioxidant activity; we used the DPPH radical reduction test according to Huang method
Results: By testing the antibacterial activity on streptococcal strains, we found inhibition diameters mean=18.5 ±2 mm (SD) using the stock solution. The one in four dilutions also had an anti-streptococcal effect with 14.5±2.3 mm for the mouthwash. This same test was carried out on the strains of S. aureus we found diameters of inhibition between 18 and 20.5 mm testing the mother solution. The C. albicans, C. glabrata, C. krusei and C. parapsilosis strains were sensitive with values between 28.5 ± 0.71mm and 36mm for the stock solution and between 9 and 11mm by testing the dilution to one out of sixteen. the DPPH radical was inhibited by the mouthwash
Conclusions: The tested mouthwash is bactericidal. In addition, we noted a very interesting anti-fungal and antioxidant activity sought from our days, especially for orthodontic patients.