IADR Abstract Archives
Asymptomatic oral Candida carriage and antifungal susceptibility profile of HIV positive patients: a case-control study
Objectives: To investigate asymptomatic oral Candida carriage rate, species distribution and antifungal susceptibility of HIV-infected patients and the association with CD4+ count.
Methods: This is a case-control study carried out in Fattouma Bourguiba Monastir University Hospital, on 35 HIV infected subjects (IG) and 35 healthy control subjects (CG) during the period from April 2014 to September 2014. An analysis of the mouth rinses samples was performed in the mycology laboratory. Strains identification was carried out by morphologic, biochemical and molecular methods (PCR). Sensibility profile was determined by E-test®. Data were analyzed by Chi square, Mac Nemar and Mann Whitney tests. A p value less than 0.05 was considered statistically significant.
Results: Oral Candida carriage rate in the IG and CG were respectively 51.4% and 20% (p=0.0002). The calculated median densities for IG and CG were 50.102 CFU/ml and 10 CFU/ml respectively (p=0.002). In the IG, 15 strains of Candida albicans, 2 C. glabrata and one association of C. albicans and C. krusei were isolated. In the CG, 6 C. albicans strains and one association of C. albicans and C. krusei were isolated. With the exception of C. krusei, which is naturally resistant to Fluconazole, all other strains were sensitive to Fluconazole and Amphotericin B. The prevalence of Candida carriage was 83.33% in the IG with CD4 + levels less than 200 CD4+/ μL (p=0.01) . An association with the carriage density and CD4 + count less than 200 CD4+/ μL was found (p=0.01).
Conclusions: In the current study, oral candidal carriage rate and density carriage are higher in HIV positive patients than in healthy individuals. Oral Candida colonization was associated with a CD4+ count less than 200/ μL. Fluconazole and Amphotericin B are efficient against strains isolated from both groups.
Methods: This is a case-control study carried out in Fattouma Bourguiba Monastir University Hospital, on 35 HIV infected subjects (IG) and 35 healthy control subjects (CG) during the period from April 2014 to September 2014. An analysis of the mouth rinses samples was performed in the mycology laboratory. Strains identification was carried out by morphologic, biochemical and molecular methods (PCR). Sensibility profile was determined by E-test®. Data were analyzed by Chi square, Mac Nemar and Mann Whitney tests. A p value less than 0.05 was considered statistically significant.
Results: Oral Candida carriage rate in the IG and CG were respectively 51.4% and 20% (p=0.0002). The calculated median densities for IG and CG were 50.102 CFU/ml and 10 CFU/ml respectively (p=0.002). In the IG, 15 strains of Candida albicans, 2 C. glabrata and one association of C. albicans and C. krusei were isolated. In the CG, 6 C. albicans strains and one association of C. albicans and C. krusei were isolated. With the exception of C. krusei, which is naturally resistant to Fluconazole, all other strains were sensitive to Fluconazole and Amphotericin B. The prevalence of Candida carriage was 83.33% in the IG with CD4 + levels less than 200 CD4+/ μL (p=0.01) . An association with the carriage density and CD4 + count less than 200 CD4+/ μL was found (p=0.01).
Conclusions: In the current study, oral candidal carriage rate and density carriage are higher in HIV positive patients than in healthy individuals. Oral Candida colonization was associated with a CD4+ count less than 200/ μL. Fluconazole and Amphotericin B are efficient against strains isolated from both groups.