Antiinflamatory Effect of Stichopus hermanii Extract as Oral Candidiasis Treatment

Objective:  Sea cucumbers have been consumed by Asian community as food and traditional medicine. Recent research stated that sea cucumber extract have its biomedical properties. Golden sea cucumber (Sticophus hermanii) extract have been known to have antifungal agent and regarding to its contents is potential to be explored as treatment in oral candidiasis. The aim of this study was to examine the anti inflamatory effect of Stichopus hermanii extract against Candida albicans in vivo. Method:  The study was an experimental laboratories research with post test only control group design. Sample were 30 male wistar rats, divided into 5 groups i.e negativecontrol, positivecontroland 3 treatment groups. Oral candidiasis condition were set up in all groups except negative control group, performed by spraying Candida albicans suspension to dorsal tongue of  wistar rats, once in 2 days for 14 days. The treatment groups were treated by Stichopus hermanii extract using feeding tube,  consecutively on the dose of  4.25 ml/kgBW, 8.5 ml/kgBW, 17 ml/kgBW. On the 14th day rats were euthanazied and the tongue were biopzied. Immunohistochemical examination was performed for anti Candida albicans antibody and TNFα, their expression were examine under light microscope. Data was analyzed by ANOVA and LSD test at 95% significance level. Result:  Stichopus hermanii extract reduced the expression of Candida albicans antibody and TNFα (p<0.05). Conclusion: Stichopus hermanii extract had the anti inflamatory effect against Candida albicans in vivo.