Antioxidant Activity of Rambutan Honey in Preventing Lipid Peroxidation

Objective: Honey is a natural substance. It is famous to help wound healing because it’s has an antioxidant, antiinflammatory, antibacterial, and debridement effects. The antioxidant substances in honey can reduce free radicals such as phenolic acids, flavonoids, ascorbic acid, carotenoids-like substances, enzymes (glucose oxidase, catalase, peroxide), and organic acids. Lipid peroxidation from free radicals is determined indirectly by measuring the levels of malondialdehyde (MDA). The objective of this research is to determine the antioxidant activity of rambutan honey in reducing the negative effect of free radicals in blood plasma oxidized by CuCl₂(in vitro). Method: The blood plasma sample was collected from an individual, selected by screening, was divided in 5 groups: blood plasma, blood plasma oxidized by CuCl_2,, plasma oxidized by CuCl₂ plus rambutan honey, plasma oxidized by CuCl₂ plus longan honey, and plasma oxidized by CuCl₂ plus vitamin C. MDA level is measured with TBARs.The research data is analyzed with Kruskal-Wallis with p<0.05 significant. Result: The results are the plasma with CuCl₂, CuCl₂with the rambutan honey group obtained significance p 0.009. Rambutan honey and vitamin C group has no significancy with p 0.602. Conclusion: In conclusion, the antioxidant activity contained in the rambutan honey can lower blood plasma level of MDA oxidized by CuCl₂ compared to the longan honey and an antioxidant activity of vitamin C.