P38 in MTA-induced Angiogenesis of Primary Human Dental Pulp Cells
Objective: To investigate mineral trioxide aggregate (MTA) influence angiogenesis of primary human dental pulp cells (hDPCs) via the MAPK pathway, in particular p38. Method: hDPCs were cultured with MTA to angiogenesis, after which cell viability, ion concentration, osmolality, NO secretion, the von Willebrand factor (vWF) and angiopoietin-1 (Ang-1) protein expression were examined. PrestoBlue®was used for evaluating hDPCs proliferation. An enzyme-linked immunosorbent assay was employed to determine vWF and Ang-1 protein secretion in hDPCs cultured on MTA and the control. Cells cultured on the tissue culture plate without the cement were used as the control. The t-test was used to evaluate the significance of the differences between the mean values. Result: MTA elicited a significant (p < 0.05) increase viability compared with the control (15%, 16%, and 13% on days 1, 3 and 5 of cell seeding, respectively). MTA consumed calcium and phosphate ions, and released more Si ions in the medium. MTA significantly (p < 0.05) increased the osmolality of the medium to 313, 328, and 341 mOsm/kg after 1 day, 3 days, and 5 days, respectively. P38 was activated through phosphorylation, and the phosphorylation kinase was investigated in the cell system after being cultured with MTA.Expression levels for Ang-1 and vWF in hDPCs on MTA were higher than those of the MTA + p38 inhibitor (SB203580) group (p< 0.05) at all of the time points. Conclusion: MTA was able to activate the p38 pathway in hDPCs cultured in vitro. Moreover, Si increased the osmolality required to facilitate the angiogenic differentiation of hDPCs via the p38 signaling pathway. When the p38 pathway was blocked by SB203580, the angiogenic-dependent protein secretion decreasesd. These findings verify that the p38 pathway plays a key role in regulating the angiogenic behavior of hDPCs cultured on MTA.
Southeast Asian Division Meeting
2014 Southeast Asian Division Meeting (Kuching, Malaysia) Kuching, Malaysia
2014 15 Scientific Groups
Lai, Wei Yun
( Chung Shan Medical University, Chung Shan Medical University Hospital, Taichung, , Taiwan
)
Huang, Shu Ching
( Chung Shan Medical University;, Taichung, , Taiwan
)
Wu, Buor Chang
( Chung Shan Medical University;, Taichung, , Taiwan
; Chung Shan Medical University Hospital, Taichung, , Taiwan
)
Kao, Chia Tze
( Chung Shan Medical University, Chung Shan Medical University Hospital, Taichung, , Taiwan
)
Shie, Ming You
( Chung Shan Medical University, Taichung, , Taiwan
)
Oral
Session 1C - Dental Materials Research
08/13/2014