Odontogenic Differentiation of hDPCs by Silicate-based Materials Stimulating

Objective: This study examines how calcium silicate (CS) and tricalcium phosphate (β­TCP) cements influence the behavior of human dental pulp cells (hDPCs) through fibroblast growth factor receptor (FGFR) and active MAPK pathways, in particular ERK. In addition, we examines how calcium silicate cement extracts influence the behavior of human dental pulp cells (hDPCs) through calcium channels and active MAPK pathway. Method: The hDPCs are cultured with β­TCP and CS, after which the cells’ viability and odontogenic/osteogenic differentiation markers are determined by using PrestoBlue® assay and western blot, respectively. In addition, cells treated with various silicon concentrations both with and without verapamil. A one-way analysis of variance statistical analysis was used to evaluate the significance of the differences between the means in the measured data. Result: The CS promotes cell proliferation and enhances FGFR. It was also found that CS increases ERK and p38 activity in hDPCs. Furthermore, raises the expression and secretion of DSP, and DMP­1. Additionally, statistically significant differences (p < 0.05) have been found in the calcium deposition in si­ FGFR transfection and ERK inhibitor between CS and β­TCP; these variations are indicated that ERK/MAPK signaling is involved in the silicon­induced odontogenic differentiation of hDPCs. Conclusion: The current study shows that silicon ions released from CS substrates play a key role in odontoblastic differentiation of hDPCs through Ca channels. And CS substrates stimulated odontoblastic differentiation of hDPCs through FGFR and modulate of ERK/MAPK activation.