Viability Neutrophil Incubated Coffee Peel Extract and Exposed Porphyromonas Gingivalis

Objective: The purpose of this research was to study the effect of coffee fruit peel extract on the viability of neutrophils exposed to Porphyromonas gingivalis. Method: The type of this research was experimental in vitro using the post test only control group design. Objects of this study were isolates of human neutrophils obtained by Ficoll hypaque centrifugation method, extract of coffee fruit peel obtained by ethanol extraction, and P. gingivalis strain ATCC 33277. The experiment was divided into 6 groups, consisting of positive control, negative control, and 4 treatment groups which were treated by the addition of the coffee fruit peel extract 25%, 50%, 75%, and 100% respectively. The procedures were as follows. Isolates of neutrophils were incubated with coffee fruit peel extract for 15 hours at room temperature (RT) and then exposed to P. gingivalis for 45 minute RT and 5% CO₂. Viability of neutrophils was observed under inverted microscope with trypan blue staining. Result: Results showed that there was significant difference between the six groups (P< 0.05). The effective concentrations to increase neutrophil viability were the addition of 100% and 75% coffee fruit peel extracts. Conclusion: Coffee fruit peel extract could improve viability of neutrophils. Further studies are needed such as clinical trials, so that it could be applied as host modulation therapy.