Methods: The transwell dentin disc model was set to simulate the clinical condition. The cytotoxicity of the GCMS extraction on 3T3 fibroblast cells and dental pulp cells were investigated by the WST-1, LDH and ALP assay with calcium hydroxide and Seal & Protect served as the comparisons and the concentration and sequence of calcium ion of GCMS , calcium hydroxide in acid condition was investigated by ion chromatography.
Results: The cytotoxicity assay revealed that GCMS extraction has good biocompatibility compared with calcium hydroxide and Seal & Protect . GCMS with 30% H3PO4 could obtain phosphoric acid in 5 minutes and start to obtain calcium ion in 10 minutes. The increasing of the phosphoric acid concentration could influence the electric property and attract calcium ion to penetrate into dentinal tubules. Moreover, the pH value was elevated and GCMS recrystalized and penetrated into dentinal tubule
Conclusions: GCMS extraction through dentin disc barrier was showed excellent biocompatibility. In acid condition, phosphoric acid attracted calcium ion to penetrate into dentin disc barrier. The GCMS with 30% H3PO4 maybe a feasible treating choice for dentin hypersensitivity clinically