Methods: Java turmeric was extracted using maseration technique with 95% ethanol and diluted with 10% DMSO. The antibacterial activity of Java turmeric against S. mutans was analyzed by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Bacterisid Concentration (MBC) using diffusion and dilution methods. S. mutans biofilm model was produced from S. mutans cultured on TYS broth for 24 hour in 37ͦC using 6 well-plate that previously layered by pelicle. The cultures were exposed to Java turmeric extract (5%-25%) for an hour, and the viability of S.mutans was analyzed using MTT assay. The effect of Java turmeric extract in inhibitting the acidogenic activity of S. mutans was analyzed by measuring the pH of 16-20 hour S. mutans biofilm after exposed to 25% extract for 1-4 hour. The Data were analyzed using Mann Whitney and ANOVA, α 0.05.
Results: Against S. mutans, the MIC of Java turmeric was 0.5% (p 0.024) and the MBC was 25% (p 0.002). Increased concentration of the extract lead to decreased viability of S. mutans. Significant decreased viability (65.65% – 17.23% compared to 100% in control) could be seen from those exposed to 0.5% - 25% extract (p 0.037). Exposure of 25% Java turmeric extract could sustained the pH of S. mutansbiofilm at 7 for 4 hours.
Conclusions: 25% Java turmeric extract is capable of inhibitting the growth, the viability and the acidogenic activity of S. mutans