The aim of this study was to investigate the effect of propolis from Thai Bees (Tetragonula laeviceps) and its component on periodontal ligament cell proliferation and cell migration.
Methods:
Periodontal ligament fibroblasts (PDL) obtained from ScienCellTM were culture in Dulbecco’s Modified Eagles Medium (DMEM) containing 10% fetal bovine serum at 37°C under 5% CO2 and 100% humidified atmosphere. Cells were treated with propolis at concentrations of 200,100,10,1,0.1 mg/ml and chrysin at concentrations of 200,100,10,1,0.1µmol. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method at day 1,4,7 and 10. To determine cell migration effect, the number of cells migrated into the scratched areas in monolayer culture was stained with toluidine blue O then counted.
Results: The results showed that both propolis from Thai bees and chrysin up to 200mg/ml and 10µmol, respectively produced no toxicity to PDL fibroblasts. It was also found that at the concentration of 10 mg/ml propolis from Thai bees and chrysin at the concentration of 1 and 0.1µmol promoted PDL fibroblast proliferation and cell migration.
Conclusions:
Our data suggested that propolis and chrysin could promote PDL proliferation and cell migration and may be good candidates for further usage in treatment of periodontal diseases.