Objectives: This study aimed to determine antibacterial activity of Holothuria atra toward Streptococcus mutans and mixed periodontopathogen, also antifungal activity toward Candida albicans.
Methods:
The experiment was done by using the post test only control group design. The antimicrobial of Holothuria atra extract to inhibit the growth of Streptococcus mutans and mixed periodontopathogen bacterial were tested by disc diffusion methods, with 3 concentrations 25mg/ml , 50mg/ml, and 100mg/ml and to Candida albicanswith 3 concentrations 10mg/ml, 20mg/ml, and 40mg/ml, each consisted of 8 samples. The inhibition effect were examined by measuring the diameter of inhibition zone. Data were analyzed by Manova, followed by LSD test.
Results:
The result of this study showed the inhibition zone around the disc of Holothuria atra extract in all concentrations. The most effective dose of Holothuria atra to inhibit S.mutans’s growth was in dose 25mg/ml with diameters 12,1012±1,4865mm (p<0,05), for mixed periodontopathogen was in dose 25 mg/ml with diameters 17,9912±1,57424mm (p<0,05), and Candida albicans was in dose 10mg/ml with diameters 8,20 ± 5,15 mm (p<0,05).
Conclusions: The effective dose of Holothuria atra extract to inhibit the growth of Streptococcus mutans and Mixed Periodontopathogen was 25mg/ml and 10mg/ml to inhibit the growth of Candida albicans