Methods: Bovine type A gelatin was incubated with ribose or methylglyoxal at a range of concentrations for 1, 3, or 7 days at 370C. Glycation was assayed by autofluorescence, percent cross-linking, and UV-Vis spectroscopy. After coating 96 well plates with the glycated gelatins or gelatin controls, HPDL cells were used for in vitro attachment, spreading, and cytotoxic assays.
Results: Autofluorescence, percent cross-linking, and UV-Vis analysis indicated a concentration dependant glycation of gelatin by both ribose and methylglyoxal. HPDL cells demonstrated a concentration dependant decrease in attachment to glycated gelatin, as well as decreased cell spreading, compared to controls. The cytotoxic assay revealed there were no adverse affects on the proliferation of the HPDL cells by either type of glycated gelatin.
Conclusions: The glycation of bovine type A gelatin was successful. Gelatin is a low cost alternative to Collagen type I, and this new glycated Gelatin/Collagen is now available for use. In vitro assays revealed HPDL cell behavior is altered on these substrates and the substrates can be used to further investigate the pathogenesis of diabetes on periodontal disease or wound healing.
This study was supported by funds from the Faculty of Dentistry, and the Petroleum and Petrochemical College, Chulalongkorn University.