Methods: Analytical expression of dentine sialophosphoprotein (DSPP) mRNA by reverse transcription polymerase chain reaction (RT-PCR). ERK1/2-MAPK was determined with U0126 (a specific inhibitor for ERK1/2-MAPK) and detected by western blot analysis.
Results: Acemannan at concentration 4 mg/ml was induces DSPP mRNA expression compared with control and induced maximum phosphorylation of ERK1/2-MAPK at 45 minute. Blockage ERK1/2-MAPK with U0126, Acemannan significantly induced phosphorylation of ERK1/2-MAPK compared with control whereas acemannan pretreated with U0126 and U0126 only are significantly suppressed phosphorylation of ERK1/2-MAPK compared with control and/or acemannan.
Conclusions: Acemannan was promoted expression of DSPP mRNA in human dental pulp cells via ERK1/2-MAPK pathway.