Methods: Before enrolled in the study, respondents fill and signature the informed consent. Twenty respondents participated as the subjects on the clinical trial , conducting two times of treatment as follows: twenty as treatment groups before and after consuming anchovy of Stolephorus insularis and the twenty subjects as control groups before and after consuming non-anchovy of Stolephorus insularis. Saliva samples were collected before and after consuming anchovy of Stolephorus insularis and with a non-anchovy of Stolephorus insularis A serial dilution was made, followed by inoculating on TYS20B medium. Data which were obtained from colony forming units of salivary mutans streptococci grew on the TYS20B medium before and after consuming anchovy of Stolephorus insularis were analyzed in a descriptive and “t” test
Results: showed that there is no significance in the average amount of Streptococcus mutans colonies between before and after consuming non-anchovy of Stolephorus insularis . However, a significant difference was found respectively as results before and after consuming anchovy of Stolephorus insularis
Conclusions:
We concluded that Anchovy of Stolephorus insularis has anti microbial activity against local strains of Streptococcus mutans isolated from human harbouring species. Therefore in a long term of consuming Anchovy fish of Stolephorus insularis , caries can be prevented