Objectives: To study the effect of partial purified royal jelly (PPRJ) on proliferation and protein expression of human gingival fibroblast (HGF) cells.
Methods: Primary HGF cell cultures were prepared and cultured with or without PPRJ. Cytotoxicity of PPRJ at 24 h and six-days proliferation of the cell cultures were determined by means of MTT and SRB methods, respectively. Total proteins from HGFs cells were extracted and identified using SDS-PAGE combined with nano-LC-MS/MS.
Results: PPRJ significantly stimulated proliferation of HGF cells. Different protein expression levels in the presence of PPRJ within 24 h were detected. They are involved in cell division, transcription, translation and metabolism.
Conclusions: PPRJ stimulated HGF cells proliferation and affected the protein expression resulting in alteration of cell function.