Methods: MTT assay was used to determined cytotoxicity of extracts to monocytes. Antibacterial activity was evaluated using disc diffusion technic. Anti-inflammation activity was determined using ELISA by quantitative measurement of TNF-α level.
Results: IC50 of crude extract and pure compounds (carbazole alkaloids) from trunk of Clausena lansium were in range of 29.38 to 120.86 µg/ml. Crude extract and some carbazole alkaloids demonstrated antibacterial activity against Porphyromonas gingivalis comparative to chlorhexidine 2%. After incubation with monocytes followed by LPS stimulation, crude extract and three carbazole alkaloids (8-formyl-6-methoxycarbazole, Lansine, Glycozolidal) showed comparative efficacy to dexamethasone in reduction of TNF-α release from monocytes.
Conclusions: Clausena lansium might provide secondary metabolites which are effective enough to further develop as drug in prevention or treatment of periodontal diseases.