Methods: Fibroblasts (HEF) derived from human embryonic stem cells (H9 cell line) were used in our experiment with human embryonic palate mesenchymal cell line ATCC CRL-1486 as a positive control. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the cDNA samples. For quantitative analysis, Osteopontin, Fibromodulin and Lumican gene expresssions were analyzed by real time RT-PCR reaction using the SYBR Green PCR Master Mix System
Results: Agarose gel analysis following RT-PCR revealed distinct expression of dermal fibroblast markers Lumican and Osteopontin. PDL fibroblast marker Fibromodulin was found depleted. Further quantitative analysis by real time RT-PCR showed that HEF had a higher expression of Osteopontin and a lower expression of Fibromodulin compared to CRL 1486.
Conclusion: The results suggest that HEF seems to resemble dermal fibroblasts.