CHITOSAN INCREASED DIFFERENTIATION OF PBMNCs INTO MSCs AND MSCs PROLIFERATION

Background: Mesenchymal stem cell (MSCs) is defined as a clonal precursor producing either identical stem cells or differentiating into other specialized cells. The success of stem cell proliferation and differentiation depends on a large extent of cell adhesion and the provision of a three-dimensional growth environment mediated by scaffold. Chitosan is natural material which has ability to biodegradability, low immunological response and positive effect on wound healing, it has been extensively used in biomedical applications. Chitosan can also increase cell adhesion. Objectives: This study is to analyze the effect of chitosan as scaffold in the proliferation of MSCs and differentiation Peripheral blood mononuclear cell (PBMNCs) into MSCs in vitro. Methods: Chitosan derived from shrimp shells and the blood samples of PBMNCs was taken from peripheral human blood. PBMNCs were seeded into a chitosan scaffold, after 7, 11 and 14 day proliferation and differentiation were analyzed with solution proliferation assay and visualized using an inverted optic microscope and scanning electron microscopy. Results: The result shows a significant level number of cells on chitosan scaffolds larger than number of cells without scaffolds (p<0.05). Conclusion: Scaffolds chitosan can increase proliferation of MSCs and differentiation PBMNCs into MSCs.