Collagen Type 1 In Direct Pulp Capping Using Chitosan

Objective: The objective of this study was to determine the increasing of synthesis collagen type I in direct pulp capping using chitosan on Rattus norvegicus for seventh and fourteenth day. Methods: Sample were molars of male Rattus norvegicus strain wistar, aged between 8-16 weeks, divided into two treatment groups, namely group I which given chitosan and group II as a control which given Ca (OH)2. Those Rattus norvegicus' occlusal molar teeth were prepared with class I cavity and then chitosan and Ca (OH)2 were each applied as the pulp capping material followed by glass ionomer cement type IX used as the restoration material. Each group then divided in two sub group i.e seven days treatment and fourteen days threatment. The teeth and jaw were then cut on the seventh day and fourteenth day followed by immunohistochemical examination which carried out to observe the synthesis of collagen type I. All data were then analyzed by t-test (α= 0,05). Result: The results showed no significant difference of synthesis collagen type I on seventh day observation (p=0,387) and there is significant difference of synthesis collagen type I on fourteenth day observation (p=0,000). Conclusion: The synthesis of collagen type I in direct pulp capping using chitosan on rattus norvegicus is higher than the one using Ca(OH)2 for fourteen days.