Plasma Protein Precipitation And Cytotoxicity Of Aluminium Sulphate Solutions

Objective: To determine the amount of plasma protein precipitation and cell cytotoxicity of three types of 25% aluminium sulphate solutions towards mouse fibroblasts. Materials and methods: Two types of 25% aluminium sulphate solutions designated as Formula B1 and B2 were developed and used in the study along with a commercially available 25% aluminium sulphate solution (GelcordTM). In vitro plasma protein precipitation was used to determine the bleeding control ability of these solutions. The percentage of protein precipitation from plasma solution was determined using protein dye binding assay. The cytotoxicity test was performed using the MTT cell viability assay on mouse fibroblast (L929). Cytotoxicity effect of B1, B2 and GelcordTM was rated according to the amount of viable cells left following exposure to each of solution for 24 hrs. Results: The plasma protein precipitation of B1 and B2 at 96.5 - 96.8% was significantly higher than GelcordTM at 53.8% (p<0.05). B1 and B2 were cytotoxic to the fibroblasts with cell viability of 21.91%, 17.86%, respectively. GelcordTM was only slightly cytotoxic producing cell viability of 76.10%. Conclusion: The plasma protein precipitation of B1 and B2 was significantly higher than GelcordTM. Hence, indicated that solutions B1 and B2 exhibited better bleeding control ability. However due to their cytotoxicity, further improvement of Formula B1 and B2 is necessary before they can be considered of clinical use.