Age and surface treatment effect on hOB reponse to implant

Introduction:Key factors for success of osseointegration are the host and the implant surface . However, little is known about the effects of aging on the responses of bone cells, especially the jaw osteoblasts.Objectives:The purpose of study is to investigate the influence of implant surface treatment and age on the differentiation/proliferation markers on human jaw osteoblasts.Materials and methods:Titanium disc with different surface.Roughness was detected by profilemeter and topography were observed by SEM.Tissue were harvested from jaw. The growth of osteoblast of different ages from human on all surfaces were compared. ALP activity were tested to determine cell differentiation at day 3,7,14 and Alarmar Blue assay were tested to determine cell proliferation at day 1,3,7,14. 15 samples were collected including 5 young samples,5 middle aged samples,and 5 old samples. Results:Osteoblasts on MAO disc or sandblasting disc looked more flat than on SLA under SEM observation. Proliferation on MAO at day 3,7,14 is the best among all sample. The proliferation in middle age group is the highest at day1,3,7,14 , but no significant difference except from the SLA group in day 3.Differentiation result is that MAO and the young at day 7,14 show better cell differentiation with significant difference.Conclusion:No significant difference at cell morphology. The MAO and middle age show better proliferation. The MAO and young are better in differentiation. So age may have some influences on the bone-implant surface interaction , means that age mat play some roles to osseointegration. But further study including more samples to get more evidence is needed.