Methods: Titanium was treated by GDP with 80W, 10min. Then fibronectin was coated on Ti surface in 4°C and 25°C, respectively. Physical properties were examined by contact angle method, surface roughness and hemocompability (OD in 540nm). Biocompatibility was determined by MTT test. All samples were observed by SEM. Differences were analyzed by T student's test (a=0.95).
Results: Ti coated fibronectin in 4°C shows lesser contact angle than 25°C. Surface roughness presents no significance difference. Test to hemocompability, fibronectin-coated Ti in 4°C presents higher OD value than 25°C. In MTT test, 25°C fibronectin-coated Ti presents higher value of cell adhesion than 4°C.
Conclusions: FTi coated fibronectin in 4°C shows better hydrophile than 25°C. Surface roughness doesn't show difference between 4°C and 25°C. Test to the hemocompability, 4°C fibronectin-coated Ti presents better OD value than 25°C. It means 4°C fibronectin-coated Ti has more blood coagulation effect. MTT test indicates that cell adhesion have significance difference between 4°C and 25°C after 48hr. It indicates 25°C fibronectin-coated Ti really has better cell adhesion than 4°C.