Methods:The expression of cyclin D1 and p27 were examined immunohistochemically on formalin-fixed, paraffin-embedded tissue sections from surgical specimens of 31 patients who had been diagnosed with OSCC of buccal and alveolar mucosa.Immunoreactivity evaluation of cyclin D1 and p27 was performed using the image analyzer (Image Pro MDA-Media Cybernetics). Statistical correlations between cyclin D1 or p27 expression and selected clinicopathological parameters of OSCC were analyzed using Fisher's exact test.
Results: Cyclin D1 positivity and reduced expression of p27 was detected in 74% and 87% of the cases respectively. Overexpression of cyclin D1 were statistically significant with larger tumour greatest dimension (T3 and T4) (p = 0.012), tumour depth > 8 mm (p = 0.043), non cohesive pattern of invasion (p = 0.001) and advanced pathological tumour stage (III-IV) (p = 0.003). Reduced p27 expression was significantly associated with non cohesive pattern of invasion (p = 0.043).
Conclusion: There was a significant correlation between overexpression of cyclin D1 and underexpression of p27 with some clinicopathological parameters which are known independent prognostic indicators. Thus, these results suggests that overexpression of cyclin D1 and underexpression of p27 may be useful as prognostic markers for OSCC of buccal and alveolar mucosa.