IADR Abstract Archives

LCMS Analysis of Centella Asiatica used for HPLF Regeneration

Objective: To identify and measure the bioactive component, asiatic acid, of Centella asiatica which can be used to enhance human periodontal ligament fibroblast (HPLF) regeneration.

Methods: A crude extract of the medicinally important Apiaceae (Centella asiatica) was purified by column chromatography. The purified compound was then analyzed by liquid chromatography-mass spectrometer (LCMS). The mass of the bioactive compound of Centella asiatica was detected using spectroscopic data.

Results: The mass spectrometric data derived by this method was sufficient for the identification and characterization of the bioactive component. The main ion m/z 488 corresponds to asiatic acid.

Conclusion: LCMS allows for a rapid and accurate measure of the active component asiatic acid in Centella asiatica, thereby providing a typical finger-print of the examined plant.

Division: Southeast Asian Division Meeting
Meeting: 2008 Southeast Asian Division Meeting (Manila, Philippines)
Location: Manila, Philippines
Year: 2008
Final Presentation ID: 92
Abstract Category|Abstract Category(s): Scientific Groups

Authors

Ahmad, Rohana ( Universiti Teknologi MARA, Shah Alam, N/A, Malaysia )

Abu Hassan, Mi ( Universiti Teknologi MARA, Shah Alam, N/A, Malaysia )

Younis, L.t. ( Universiti Teknologi MARA, Shah Alam, N/A, Malaysia )

Said Ghulam Khan, Hb ( Universiti Teknologi MARA, Shah Alam, N/A, Malaysia )

Khor, Gh ( Universiti Teknologi MARA, Shah Alam, N/A, Malaysia )

Abdul Majeed, Ab ( Universiti Teknologi MARA, Shah Alam, N/A, Malaysia )

SESSION INFORMATION

Poster Session
Poster Presentation
10/10/2008