Objectives:To measure IgA level in patients with amalgams restoration and to compare IgA levels between gender.
Methods: Saliva from 54 FKGUI students was collected and divided into four groups: male with amalgam restoration (MA)group (18 students), female with amalgam restoration(FA)(25 students), male control(MC)group(4 students),female control(FC)group(7 students.
Amalgams restorations group criteria are having one/more amalgams fillings, no other filling material,no periodontal or any systemic diseases
Control group criteria are free caries,no periodontal or systemic diseases.
Sample preparation : Ten mililiter saliva was collected from the subjects, placed into tubes and centrifuged at 200 rpm for 15 minutes. Supernatan were collected and then freezed. Sample was dialyzed with Phosphate Buffer Saline solution (PBS) for an hour, five times with new PBS solution, after that IgA level was measured with Turbitimer (Behring) and IgA level was recorded. Statistical analysis was made between groups and gender using Student's t-test,
Results: No significant difference is between the IgA level of MC group (54.2 ± 4.24 IU/ml) and FC group (55.06 ± 7.31 IU/ml). But, the IgA level in MA group (86.33 ± 11.06 IU/ml) and in FA group 82.65 ± 6.37 IU/ml are significant differences from that in MC group and FC group, respectively. IgA levels between male and female in control group and amalgam group are not different.
Conclusions: IgA level in amalgam restoration group significantly increased as compared to the control group. This result showed amalgams affect the immune response.