Effects of Cissus Quadrangularis on osteoblastic MC3T3-E1 cells

Although many studies have noticed the systemic effects of Cissus quadrangularis (CQ) during bone recovery process, its mechanism of action on osteoblasts is yet unknown. Objectives: In this study, the influence of CQ on osteoblastic MC3T3-E1 cell viability and alkaline phosphatase (ALP) activity were measured. Methods: After treated with 0.01% to 0.5% w/v of CQ extract in culture for 21 days, MTT assay and ALP activity were investigated. Results: The lower concentrations of CQ extract (0.01%-0.15%) had no cytotoxic effect throughout the observation period, while the 0.25% and 0.5% CQ extract concentrations were toxic from day 14 onwards. Treatment of 0.01% w/v CQ to MC3T3-E1 significantly enhanced ALP activity from day 5 to day 21 of culture. Conclusions: The 0.01% CQ extract increases ALP activity of MC3T3-E1 without cytotoxicity effect.