GCF and Salivary Level of ALP in PeriodontitisSmokers and Non-smokers

OBJECTIVES: Periodontitis has an episodic pattern with periods of activity (destruction) and quiescence. It is therefore important to diagnose it in active phase. Different markers have been identified from Gingival Crevicular Fluid (GCF) and saliva. The level of these markers could be used as indicators of disease activity. Alkaline phosphatase is one of important markers of bone loss. Many cross sectional studies have shown the relation between raised ALP level of GCF and periodontitis. Besides, smoking is a major risk factor for occurrence and severity of periodontitis. Therefore, the aim of this research was to measure GCF and salivary levels of ALP in healthy and periodontitis patients. The level of ALP was further measured in periodontitis smokers and periodontitis non- smokers. METHODS: 90 patients aged 35-90 without any systemic disease was equally divided into 3 groups: 1-Periodontitis and smokers 2-Periodontitis and non-smokers 3-Healthy. In 1st and 2nd groups GCF samples were collected from the 5-7 mm deep pockets. In 3rd group sample was collected from healthy gingival sulcus. The 5 ml of unstimulated saliva was collected from all samples. Then, the level of ALP in GCF and saliva was measured by ELISA. Results: The mean level of ALP was significantly higher in GCF than saliva in all subjects. The mean level of ALP in GCF and saliva was significantly higher in periodontitis subjects. The mean level of ALP in GCF was significantly higher in periodontitis smokers Than non-smokers periodontitis subjects . CONCLUSION: ALP level in GCF and saliva could be an important marker of periodontis which is higher in smokers.