The Effect of S. spontaneum L. on Rat's Heart

Previous reserach has revealed that S. spontaneum L. could be used as a bleaching agent for lighten the external tooth discoloration. However, the safety level of S. spontaneum L. has not yet been established, especially to the vital organ. Objectives: The acute toxicity test of white Wistar rat's heart had been done to prove the safety level of S. spontaneum L. Methods: Twenty four Wistar rats were used and devided into eight groups. The treated groups were given extracts with dosages of 0.5g/kgBW, 5g/kgBW and 15g/kgBW of the S. spontaneum L. orally, and were killed after 2 hours and 24 hours. The control group was also killed after two hours and 24 hours after applications with aquadest. The tissue specimens were histologically prepared,stained with HE, and investigated under light microscope. Results: The Chi-square statistical test shows that X² calculation of nuclear and myocardium fiber hypertrophy (X²=11.5000) less than X² table (X²=12.017). Meanwhile the X² of inflamed cells (X²=13.723) less than X² table (X²=14.067). Conclusions: S. spontaneum L extract did not show any toxic effect to white Wistar rat's heart after 2 and 24 hours intake.