Music, Protein-Deficiency, and Enamel-Mineral Content in Rat-Pups

Objectives: To analyze the effect of music and post natal isocaloric protein deficiency on the percentage of N, Calcium (Ca), and Phosphor (P) contents on the incisor-enamel of 7 day-old rat-pups. Methods: Experimental female Wistar rats were exposed to daily classic music since gestation period day 1 until the pups were terminated, controls had no music. Since the pups were 2 days old, the animals were differed into normal (19.5% protein) and protein-deficiency (7.5% protein) groups, fed ad libitum. The 6 pups of each group were randomly terminated at day 7. The percentage of N, Ca, and P content on the surface of incisor-enamel were analyzed using Energy-Dispersive-X-Ray method (EDX). ANOVA with α=0.05 were used. Results: Isocaloric protein deficiency on 7 day old pups did not result in significant decreased of N, Ca, and P contents of enamel either in groups with or without music. There was significant differences of N content in protein-deficiency groups between animals with (34.09 %) and without music (41.95%) [p=0.013]. In addition, there was significant differences of Ca content in normal diet groups between animals with (5.69%) and without music (2.10%) [p=0.016]. And there was significant differences of P content in normal diet groups between animals with (7.45%) and without music (1.43%) [p=0.001]. The enamel-Ca and -P contents of normal diet animals with music were also significantly higher than those with protein-deficiency and without music, p<0.05. Conclusions: Postnatal isocaloric protein deficiency in 7 days old pups did not result in decreased enamel-N, -Ca, and -P contents. However, in pups with protein-deficiency music-exposure could lead to lower enamel N-content, and in pups with normal diet music resulted in higher Ca- and P-content.