Detection Of Impedance Characteristics Of Periodontalcells Using Biochips

Objectives: Electrical impedance sensing (EIS) has been used for measuring the dynamic behavior of various cells. However, its application in dental research was not seen. Since research on periodontal disease is an important issue in modern dentistry, developing an EIS device suitable for periodontal cells detection is valuable. The aim of this study is to develop a novel biochip based on EIS theory for periodontal cells detection. Methods: In this study, a biochip with indium tin oxide electrode was developed. MG-63, MC3T3-E1 and NIH 3T3 cells were carried out as test subjects. The EIS were performed on all the three cells using the newly-developed biochip. Besides, the impedance changes of the cells were detected when the cells were co-cultured with toxicants, such as LPS and LTA. Results: Our preliminary results demonstrated that cells with higher density revealed large values of electrical impedance difference. In contract, damaged cells showed related low values in impedance due to round up. Conclusions: A novel method based on EIS was established in this study. It is sensitive for detecting cell behaviors without damage the cell structure chemically and physically. It can be served as a useful tool for the periodontal studies.