Static Magnetic Fields Stimulate Osteoblastic Cells Growth on PolyLactide Surface

Objective: Bone graft substitutes have been widely used to promote large bony defects healing. These bony defects were usually caused due to accident-induced craniomaxillofacial injury or tumor resection. Bioresorbable polymer [Poly(L-lactide), PLLA] scaffolds are frequently used in the rehabilitation of craniomaxillofacial defects owing to its biodegradability and biocompatibility. Geometrical and chemical improvements were two major strategies to improve cell growth on the PLLA surface. However, these methods may have some disadvantages of the lower cell proliferation and attachment rate. To improve the osteoblast-like cells growth on the PLLA surface, static magnetic fields were non-invasively applied to MG63 cells. Methods: The MG63 cells were continuously exposed to 4000 Gauss-SMF for 5 days. Scanning electron microscopy studies indicated that SMF promoted MG63 cell express extracellular matrix on the PLLA surface. Results: After 2 and 3 days, SMF exposed cells exhibited decreased MTT values. At 1 day, the alkaline phosphatase-specific activity of the SMF exposed cells demonstrated a maximum level and significantly larger than that of the unexposed cells (p<0.05) with an increasing ratio of 1.5 folds. Conclusions: MG63 cells, seeding on the PLLA surface, treated with SMF exhibit a more differentiated morphology and express a more differentiated regulatory marker.