Measurement of Alkaline Phosphatase in Osteoblast Activity in Coral Scaffold

Coral goniopora, as an osteoconductive synthetic bone subtitute material can be found in Indonesian ocean. The microstructure of Coral goniopora is similar to cancellous bone, contains calcium carbonate with total porosity >70%, pore size 500-600µm, and interconnecting pores 220-260µm. Objective: the aim of this study was to evaluate the effect of Coral goniopora application on human osteoblast cell line MG63 activity by measuring the concentration of alkaline phosphatase content in the cell culture supernatant. Method: Osteoblasts were cultured in a-MEM medium supplemented with 10%FBS, 300 ng/ml fungizone and 1% Penicillin-Streptomycin under standard cell culture condition for 48 hours. Then osteoblasts were seeded at a density of 100.000 cells onto 2 mg Coral goniopora with different sizes: A(20 mesh), B(1-2mm), C(200 mesh). After 24 and 48 hours, alkaline phosphatase (ALP) content in cell culture supernatants were measured by using colorimetric technique. Data obtained was analyzed by ANOVA. Results: we showed that there was no significant difference in ALP concentration after 24 hours. Nevertheless, there was significant difference in ALP concentration in C size coral application in stable culture comparison with A and B at 48 hours. Conclusion: We concluded that the time of culture and the coral size will affect the osteoblast activity in vitro with ALP as specific bone regeneration indicator.