Effect of Penstrep and Fungizone towards Dengue Virus

Background: Dengue virus and Human Immunodeficiency Virus Dengue are RNA viruses. We known that HIV as AIDS causing is susceptibility towards some antifungi agents such as fungizone. It did not surprise if prove the evidence that HIV transmission rate via saliva much less small compared by blood. However there are 300 microorganism species in our oral cavities, there are natural anti fungi in saliva such as mucin. Objectives: The aim of this research is to study the effect of penstrep and fungizone against Dengue viruses. Material and methods: The saliva monocyte cells were isolated from Dengue Haemorrhagic Fever patients (that confirmed with PCR) and normal human and cultured in a FBS + RPMI medium with 5% IL-2. Cells were treated with a mixture of Penstrep and Fungizone with different ratio 1:1; 1:2; 2:1. The cultures were observed by inverted microscope. From the microscope we can see the intact saliva monocyte cells and the giant cells that contained the immature Dengue viruses. Result:The Dengue virus was proved to be survival in group with 2% Penstrep and 1% Fungizone. Conclusion:Such data could contribute to the research development towards Dengue infection especially in our saliva.