Biofilm Microbiological Variations in Dental Units of Oral Surgery Clinic

Infectious disease often occur in Indonesia .These infections are widely known that most bacteria found scattered in the natural environments exist in the form of communities, which are commonly referred to as biofilms. Biofilms reservoirs are microorganisms natural tendency in communities altering the water quality that circulates through dental unit water lines. Objectives: The aim of this study was to determine microbiological changes in dental units water after 72 hrs at rest. Methods: The samples were obtained from ten water samples were from 10 dental units of Oral Surgery Clinic in Jakarta and taken 10 ml of water of each dental units in sterilized glass bottle . A 10 ml water sample diluted serially tenfold . An aliquot of 0.1ml of diluted sample was placed on Agar Blood medium plate and incubated in an anaerobic chamber at 37 Celsius for 72 hours. Isolated samples were examined for colony and cellular morphological differentiation, Gram staining characteristic and biochemical tests. Data obtained was done in descriptive method. Results: showed that cocos Gram + (negative coagulasa) = 8000 colonies /ml of water , Bacillus Gram – (not fermentation) = 12000 colonies of water and filaments = 6000 colonies of water. The CFU that grew on the Agar blood medium that consists of Pseudomonas aeruginosa are higher in number than Streptococcus haemoliticus ,Staphylococcus aureus , Fusobacterium spp Diptheroid and Streptococcus epidermidis Conclusion: Samples taken from dental units water at rest for 72 hrs were heavily contaminated with bacteria. During rest period there a mark saprobious microbial proliferation It can detected the presence of species characteristic of stagnant water this may be represent a potential infection risk to immunocompromised dental patients. This suggests that the dental units water lines system should be disinfected with high concentrate disinfectant routinely