Responses of primary osteoblasts and MG-63 to pulsed electromagnetic field

Objective: Investigate the effect of pulsed electromagnetic field (PEMF) on cellular proliferation and osteocalcin production of osteoblast-like cell line, MG-63 cells, and human normal osteoblast cells (NHOC) obtained from surgical bone specimens. Methods: The cells were placed in 24-well culture plates in the amount of 3x10⁴ cell/wells with 2 ml DMEM media supplemented with 10% FBS. The experimental plates were placed between a pair of Helmoltz coils powered by a pulse generator (PEMF, 50 Hz, 1.5 mV/cm) in the upper compartment of a dual incubator (Forma). The control plates were placed in the lower compartment of the incubator without Helmotz coils. After three days, the cell proliferation was analyzed by the method modified from Mossman (J. Immunol Methods 1983; 65: 55-63). Other sets of plates were used for osteocalcin production assessment. Media from these sets were collected after 6 days and assessed for osteocalcin production using ELISA kits. The data were analyzed using one-way analysis of variance (ANOVA). Results: MG-63 cells from the experimental group proliferated significantly more than those from the control group (p < 0.05). No significant difference in osteocalcin production was detected between the two groups. On the other hand, NHOC from the experimental group produced larger amount of osteocalcin (p < 0.05) than those from the control group. However, no significant difference in cell proliferation was detected between the two groups. Conclusion: PEMF effect on osteoblasts might depend on their cell type of origin. For osteoblast-like cell line, MG-63 cells, PEMF increased proliferation rate but not osteocalcin production of the cells. However, PEMF stimulation effect on human normal osteoblast cells was most likely associated with enhancement of osteocalcin production, but not with the increase in cell numbers. Supported by PSU grant 0638/46