Previous work has showed that factory ready made dentifrices containing triclosan, baking soda or amyloglucooxidase and glucooxidase enzymes can significantly reduce oral Streptococci mutans. Objectives: This study aimed to investigate the effect of toothbrushing with dentifrice containing triclosan, baking soda, or amyloglucooxidase and glucooxidase enzymes on the population of anaerobic Gram-negative bacteria (rods). Methods: Forty participating students of the University of Indonesia were treated as follows: the test group was instructed to brush their teeth with dentifrice containing triclosan, baking soda, or enzyme. The control group was similarly treated but by using standard dentifrice without these ingredients. Gargle samples containing 20ml of 2.5% NaCl were taken from each subject before and after treatment, diluted directly in 9ml of 2.5% NaCl, and inoculated onto blood agar plate. The plates were incubated in an anaerobic jar for 48-72 hours at 37°C. The Colony Form Unit (CFU) data obtained from the observed bacterial populations were analyzed with GLM (repeated measures). Results: The CFU values of various treatment and control groups before and after were shown in the following table:
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| Triclosan | Baking Soda | Enzyme | |||
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| Treatment | Control | Treatment | Control | Treatment | Control |
| Before | 275.42±2.4 | 239.88±3.02 | 107.15±4.07 | 177.83±2.04 | 144.54±4.17 | 363.08±2.88 |
| After | 89.13±3.8 | 167.82±2.57 | 43.65±5.37 | 64.57±2.88 | 47.86±6.03 | 95.5±4.07 |
| p value* | p=0.03 | p=0.001 | p=0.006 | |||
* p values of treatment vs. control group
Conclusion: The dentifrice containing triclosan, baking soda or amyloglucooxidase and glucooxidase enzymes significantly reduced the anaerobic Gram negative-bacteria (rods).